Objective: To evaluate the knowledge, attitude, and preparedness of Mahabad Red Crescent Society volunteers in dealing with chemical attacks.Methods: This prospective cross-sectional study was conducted on 120 Red Crescent Society volunteers in Mahabad City, Iran, during 2014-2015.The knowledge of the volunteers was evaluated and rated using a questionnaire as poor, moderate, and good. Also, the attitude of the volunteers towards the chemical attacks and their preparedness were rated as appropriate and inappropriate using a questionnaire. Data were analyzed using the SPSS software version 21. Results: From a total of 120 volunteers, 62.5% were males. The mean age of the volunteers was 32.0 ± 8.2 years. None of the volunteers had adequate knowledge regarding management of the consequences of chemical terrorist attacks. Only 10 volunteers (8.3%) had appropriate attitude and 7 (5.8%) stated their preparedness for being sent to the crisis zone. Also, 116 volunteers (96.7%) declared that Mahabad Red Crescent Society has an inappropriate level of preparedness to encounter chemical terrorism attacks and release of chemical agents related to petrochemical industrial chlorine resources into the water and wastewater. Conclusion: The findings of the present study show poor knowledge and inappropriate attitude of Mahabad Red Crescent Society volunteers, and rescuers in encountering probable chemical attacks and industrial accidents. Furthermore, the Red Crescent Society had an inappropriate level of preparedness in the field of chemical terrorism from the viewpoint of the studied volunteers.