Objective: To determine bilateral effects of unilateral iatrogenic vas deferens trauma (UIT) on epididymal sperm characteristics and in vitro fertilizing capacity in an experimental mouse model.Methods: Experiments were performed on three equal groups each comprising six adult male mice. Following anaesthesia, UIT was induced by clamping left vas deferens with a mosquito clamp in fully locked fashion for 2 minutes in UIT group. Control-sham mice only had exposure of the left vas deferens through a groin incision. Control animals only received ceftriaxone (100 mg/kg) intraperitoneally at the day of experimental UIT induction. Ipsilateral and contralateral epididymal sperm characteristics and in vitro fertilizing capacity were evaluated after 35 days.Results: UIT significantly decreased sperm concentration, motility and viability as well as fertilization, two-cell embryos, blastocysts and hatched blastocysts rates. Moreover, incidence of DNA damage and abnormality in spermatozoa was significantly higher in UIT group.Conclusion: The findings suggest that a non-recognized iatrogenic vas deferens trauma may have detrimental effects on spermatozoa leading to infertility.
Babaei, M., Najafi, G., Shalizar Jalali, A. & Behfar, M. (2015). Effects of Unilateral Iatrogenic Vas Deferens Trauma on Fertility: An Experimental In Vitro Fertilization Mice Model Study. Bulletin of Emergency And Trauma, 3(Issue 4), 122–127.
MLA
Babaei, M., Najafi, G., Shalizar Jalali, A., & Behfar, M. "Effects of Unilateral Iatrogenic Vas Deferens Trauma on Fertility: An Experimental In Vitro Fertilization Mice Model Study", Bulletin of Emergency And Trauma, 3, Issue 4, 2015, 122–127.
HARVARD
Babaei, M., Najafi, G., Shalizar Jalali, A., & Behfar, M. (2015). 'Effects of Unilateral Iatrogenic Vas Deferens Trauma on Fertility: An Experimental In Vitro Fertilization Mice Model Study', Bulletin of Emergency And Trauma, 3(Issue 4), pp. 122–127.
CHICAGO
Babaei, M., Najafi, G., Shalizar Jalali, A. & Behfar, M., "Effects of Unilateral Iatrogenic Vas Deferens Trauma on Fertility: An Experimental In Vitro Fertilization Mice Model Study." Bulletin of Emergency And Trauma, 3 Issue 4 (2015): 122–127,
VANCOUVER
Babaei M., Najafi G., Shalizar Jalali A., Behfar M. Effects of Unilateral Iatrogenic Vas Deferens Trauma on Fertility: An Experimental In Vitro Fertilization Mice Model Study. Bull Emerge Trauma. 2015;3(Issue 4):122–127.
