Fariborz Ghaffarpasand; Mostafa Shahrezaei; Maryam Dehghankhalili
Volume 4, Issue 3 , July 2016, , Pages 134-140
Abstract
Objective: To determine the effects of platelet rich plasma PRP on healing rates of long bone non-union fracture.Method: This was a randomized double-blind placebo controlled clinical trial being performed in a 12-month period. We included 75 adult (>18 years) patients suffering from long bone (Femur, ...
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Objective: To determine the effects of platelet rich plasma PRP on healing rates of long bone non-union fracture.Method: This was a randomized double-blind placebo controlled clinical trial being performed in a 12-month period. We included 75 adult (>18 years) patients suffering from long bone (Femur, Tibia, Humerus and Ulna) non-union fracture who were randomly assigned to receive 5mL PRP (n=37) or 5mL normal saline as placebo (n=38) in the site of fracture after intramedullary nailing or open reduction and internal fixation (ORIF) along with autologous bone graft. Patients were followed each 45 days till 9 months and were evaluated both clinically and radiologically in each visit. The healing rate, failure rate, incidence of infection, mal-union and limb shortening were recorded and compared between groups after 9 months of follow-up.Results: The healing rate was significantly higher in PRP group compared to placebo (81.1% vs. 55.3%; p=0.025). The limb shortening was significantly higher in those who received placebo (2.61±1.5 vs. 1.88±1.2mm; p=0.030). Injection of PRP was also associated with lower pain scores (p=0.003) and shorter healing duration (p=0.046). The surgical site infection (p=0.262) and mal-union rate (p=0.736) were comparable between groups.Conclusion: Application of PRP along with autologous bone graft in the site of non-union of long bone after intramedullary nailing or ORIF results in higher cure rate, shorter healing duration, lower limb shortening and less postoperative pain. Higher infection rate might be a complication of PRP application.Keywords: Non-union; Long bone Fracture; Platelet rich plasma (PRP); Intramedullary nailing; Open reduction and internal fixation (ORIF).Clinical Trial Registry: This trial is registered with the Iranian Clinical Trials Registry (IRCT201208262445N1; www.irct.ir).
Rahim Mohammadi; Moein Mehrtash; Moeid Mehrtash; Nava Hassani; Ali Hassanpour
Volume 4, Issue 1 , January 2016, , Pages 29-37
Abstract
Objective: To assess the effects of platelet rich plasma (PRP) with chitosan biodegradable film on full thickness wound healing in rat.Methods: This was an experimental study being performed in 2015 during a 4-month period. Twenty-four male white Wistar rats were divided into four groups of 12 rats each, ...
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Objective: To assess the effects of platelet rich plasma (PRP) with chitosan biodegradable film on full thickness wound healing in rat.Methods: This was an experimental study being performed in 2015 during a 4-month period. Twenty-four male white Wistar rats were divided into four groups of 12 rats each, randomly: Control group (SHAM) with creation of wounds and no treatment, PRP group with creation of wounds and application of one milliliter PRP, Chitosan group (CHIT) with dressing the wound with chitosan and CHIT/PRP group with application of one mL PRP and dressing the wound with chitosan. The wounds were created by cutting healthy skin. Wound size was measured on 6, 9, 12, 15, 18 and 21 post surgery and was compared between groups.Results: Reduction in wound area, hydroxyproline contents and biomechanical parameters indicated there was significant difference (p=0.001) between group CHIT/PRP and other groups. Biomechanical testing was performed on day 9 post surgery in incisional model. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (p<0.001) between group CHIT/PRP and other groups.Conclusion: PRP with chitosan have beneficial effects on wounds repair and could be suggested for treating various types of wounds in animals and human being.
Sedighe Abbasipour-Dalivand; Rahim Mohammadi; Vahid Mohammadi
Volume 3, Issue 1 , January 2015, , Pages 1-7
Abstract
Objective: To determine the effects of local administration of platelet rich plasma (PRP) on peripheral nerve regeneration in rat sciatic nerve transection model.Methods: Forty-five male white Wistar rats were randomized into three experimental groups (n = 15): Normal control group (NC), silicon group ...
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Objective: To determine the effects of local administration of platelet rich plasma (PRP) on peripheral nerve regeneration in rat sciatic nerve transection model.Methods: Forty-five male white Wistar rats were randomized into three experimental groups (n = 15): Normal control group (NC), silicon group (SIL), PRP treated group (SIL/PRP). In NC group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In SIL group left sciatic nerve was exposed the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone conduit and filled with 10 µL phosphate buffered solution. In SIL/PRP group silicon conduit was filled with 20 µL PRP. Each group was subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks after surgery.Results: The animals were comparable regarding the baseline characteristics. Behavioral testing, sciatic nerve functional study and gastrocnemius muscle mass showed earlier regeneration of axons in SIL/PRP than in SIL group.Conclusion: Local administration of PRP combined with silicon grafting could accelerate functional recovery of peripheral nerve. Easily available growth factors and bioactive proteins present in PRP may have clinical implications for the surgical management of patients after nerve transection.
